Studying all the cells in humans is an enormous endeavor—current estimates suggest human bodies are made of at least 35 trillion cells. Among the spaces in the body, the oral and craniofacial tissues are incredibly diverse, including tongue to teeth and gums to glands. Each is supported by epithelia, cartilage, bone, ligaments, muscles, adipose tissue, blood and lymphatic vessels, and nerves; and all of these tissues are harmoniously integrated into the vital functions of communication, feeding, breathing, defense, sensing, and early digestion. Oral and craniofacial tissues are affected in many disorders and diseases that can decrease quality of life and cause deleterious psychosocial issues, emphasizing the need for effective, precise, and aesthetic strategies for the regeneration of craniofacial tissues. New approaches in single-cell omics are already allowing for comprehensive and integrated cell atlases to understand the cell types that support these niches in health and uncover which cell types and networks are affected in disease. This level of cell annotation and integration will be critical for understanding oral and craniofacial diseases across the lifespan. In tandem, due to the growing body of literature displaying interconnected roles for oral and systemic health, findings from this network will be critical to future meta-analyses with other tissue and organ atlases for precision diagnostics and treatments. This session will feature uses of these new single-cell approaches to resolve our collective biology at a higher resolution and will also outline future directions for mapping the oral and craniofacial tissues across the lifespan.