2021 IADR/AADR/CADR General Session
The 99th General Session & Exhibition of the IADR was to be held in conjunction with the 50th Annual Meeting of the American Association for Dental Research and the 45th Annual Meeting of the Canadian Association for Dental Research, from July 21-24, 2021 as a Virtual Experience.The recordings in this library from the meeting are a selection of the science that was to be presented as part of the General Session. These recordings give you the opportunity to participate in the meeting and hear from leading researchers. The recordings include IADR Distinguished Lecture Series speakers, the IADR Academy and symposia from a collection of scientific groups and networks.
This session can be purchased as part of the full meeting recordings within the product bundles
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Session Description
An examination of the extremes of dental regenerative potential across the animal kingdom provides a path towards human tooth regeneration. The majority of dental stem cell research has been focused on mesenchymal cells that might have the potential to form dentin and tooth attachment tissues. But in order to form crowns and roots we need to investigate the epithelial stem cell population. In this symposium we will discuss mammalian and reptilian animal models as well as the potency of adult dental epithelial stem cells in humans.
The speakers will demonstrate that the multipotent epithelial stem cell is conserved across vertebrates and that remnants of human dental epithelium can form enamel in pathological conditions. Together this symposium will provide fascinating insights the location and potential ways to harness the regenerative potential of dental epithelial stem cells. Reseseach on epithelial and mesenchymal stem cells from developmental biology and pathological contexts will bring us closer to the ultimate goal of full tooth bioengineering (crowns plus roots). To start the symposium Dr. Chiba will introduce the dental epithelium and the expression profile of individual cells isolated from the mouse incisor.
The elegant in vivo work identified a cluster of epithelial cells genetically marked with Krt14-RFP. However to increase recovery of healthy cells, the authors did not use flow cytometry to separate the RFP cells prior to scRNAseq. This permitted a deep transcriptome profiling of many layers of the enamel organ including the stellate reticulum and stratum intermedium that house epithelial stem cells. This work has revealed genes previously not associated with tooth development that may be required for life-long stem cell maintenance. Dr. Richman will chair the session and the co-chair will be Dr. Balic. Dr. Richman will be discussing the tooth replacement process in reptiles in which continuous tooth formation is maintained throughout the life of the animal. In lizards and snakes, adult epithelial stem cells are required for normal tissue homeostasis. Her talk will describe the fascinating removal of second generation teeth in the gecko and how the remaining dental lamina reboots tooth formation. The labeling of epithelial cells with BrdU offers a fascinating window into the process of converting stem cells into different lineages in the enamel organ.
Dr. Richman will present some new single-cell RNAseq data that profiles expression in dental epithelial cells of the gecko. These data will be linked to other data from the mouse incisor and human dental epithelial cancers. Dr. Heikinheimo will presenting her work defining the molecular characteristics of human ameloblastoma and the conserved gene expression shared with classic stages of tooth development. The origins of the ameloblast-like cells will be discussed as well as the progression from normal epithelium to the cancerous state. Her findings highlight that remnants of the dental epithelium such as the dental lamina near 3rd molars retain odontogenic potential into early adulthood. The deep understanding of the oncology of ameloblastoma will help to steer tooth bioengineering towards normal rather than abnormal development.
Dr. Balic will co-chair the session with Dr. Richman. Dr. Balic is the first to culture dental epithelial stem cells in spheroids from the mouse model. Her work showed that SOX2-GFP can be used to sort out epithelial cells with flow cytometry and that these same cells can be grown under conditions that will one day lead to amelogenesis. This is very exciting work that gets at the practicalities of expanding dental epithelial cells for future use in tissue engineering.
Learning Objectives
July 21, 2021
Presenters
Yuta Chiba - Single-Cell RNAseq Profiling of the Dental Epithelium in the Ever-Growing Mouse Incisor
Joy Richman - Dental Epithelial Stem Cells Give Rise to New Teeth in Vivo in the Lizard
Anamaria Balic - Expansion of Dental Epithelial Stem Cells From the Adult Mouse Incisor
Kristiina Heikinheimo - The Relationship Between Ameloblastoma and Adult Dental Epithelial Stem Cells
Sponsoring Groups/Networks
Craniofacial Biology Research
CE Credits
1.5 Contact Hours
Financial Interest Disclosure:
The speakers are all working at academic institutions and do not have financial conflicts of interest. Yuta Chiba, Department of Oral Health and Development Sciences, Tohoku University Graduate Schoolof Dentistry, Sendai, JapanKristiina Heikinheimo - Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Turku and Turku University Hospital, FinlandAnamaria Balic - Research Program in Developmental Biology, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandJoy Richman - Faculty of Dentistry, University of British Columbia, Vancouver BC, Canada
An examination of the extremes of dental regenerative potential across the animal kingdom provides a path towards human tooth regeneration. The majority of dental stem cell research has been focused on mesenchymal cells that might have the potential to form dentin and tooth attachment tissues. But in order to form crowns and roots we need to investigate the epithelial stem cell population. In this symposium we will discuss mammalian and reptilian animal models as well as the potency of adult dental epithelial stem cells in humans.
The speakers will demonstrate that the multipotent epithelial stem cell is conserved across vertebrates and that remnants of human dental epithelium can form enamel in pathological conditions. Together this symposium will provide fascinating insights the location and potential ways to harness the regenerative potential of dental epithelial stem cells. Reseseach on epithelial and mesenchymal stem cells from developmental biology and pathological contexts will bring us closer to the ultimate goal of full tooth bioengineering (crowns plus roots). To start the symposium Dr. Chiba will introduce the dental epithelium and the expression profile of individual cells isolated from the mouse incisor.
The elegant in vivo work identified a cluster of epithelial cells genetically marked with Krt14-RFP. However to increase recovery of healthy cells, the authors did not use flow cytometry to separate the RFP cells prior to scRNAseq. This permitted a deep transcriptome profiling of many layers of the enamel organ including the stellate reticulum and stratum intermedium that house epithelial stem cells. This work has revealed genes previously not associated with tooth development that may be required for life-long stem cell maintenance. Dr. Richman will chair the session and the co-chair will be Dr. Balic. Dr. Richman will be discussing the tooth replacement process in reptiles in which continuous tooth formation is maintained throughout the life of the animal. In lizards and snakes, adult epithelial stem cells are required for normal tissue homeostasis. Her talk will describe the fascinating removal of second generation teeth in the gecko and how the remaining dental lamina reboots tooth formation. The labeling of epithelial cells with BrdU offers a fascinating window into the process of converting stem cells into different lineages in the enamel organ.
Dr. Richman will present some new single-cell RNAseq data that profiles expression in dental epithelial cells of the gecko. These data will be linked to other data from the mouse incisor and human dental epithelial cancers. Dr. Heikinheimo will presenting her work defining the molecular characteristics of human ameloblastoma and the conserved gene expression shared with classic stages of tooth development. The origins of the ameloblast-like cells will be discussed as well as the progression from normal epithelium to the cancerous state. Her findings highlight that remnants of the dental epithelium such as the dental lamina near 3rd molars retain odontogenic potential into early adulthood. The deep understanding of the oncology of ameloblastoma will help to steer tooth bioengineering towards normal rather than abnormal development.
Dr. Balic will co-chair the session with Dr. Richman. Dr. Balic is the first to culture dental epithelial stem cells in spheroids from the mouse model. Her work showed that SOX2-GFP can be used to sort out epithelial cells with flow cytometry and that these same cells can be grown under conditions that will one day lead to amelogenesis. This is very exciting work that gets at the practicalities of expanding dental epithelial cells for future use in tissue engineering.
Learning Objectives
- To explain the characteristics of an adult tissue resident stem cell in terms of marker expression and proliferation profiles
- To describe the typical locations of epithelial stem cells in teeth and the conditions that support maintenance of dental epithelial stem cells in reptiles, mice and humans
- To explain the lab-based and clinical evidence that suggests adult dental epithelial stem cells have the potential to form ameloblasts.
July 21, 2021
Presenters
Yuta Chiba - Single-Cell RNAseq Profiling of the Dental Epithelium in the Ever-Growing Mouse Incisor
Joy Richman - Dental Epithelial Stem Cells Give Rise to New Teeth in Vivo in the Lizard
Anamaria Balic - Expansion of Dental Epithelial Stem Cells From the Adult Mouse Incisor
Kristiina Heikinheimo - The Relationship Between Ameloblastoma and Adult Dental Epithelial Stem Cells
Sponsoring Groups/Networks
Craniofacial Biology Research
CE Credits
1.5 Contact Hours
The speakers are all working at academic institutions and do not have financial conflicts of interest. Yuta Chiba, Department of Oral Health and Development Sciences, Tohoku University Graduate Schoolof Dentistry, Sendai, JapanKristiina Heikinheimo - Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Turku and Turku University Hospital, FinlandAnamaria Balic - Research Program in Developmental Biology, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandJoy Richman - Faculty of Dentistry, University of British Columbia, Vancouver BC, Canada
