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Description
The IASP 2022 World Congress on Pain took place from 19-23 September 2022, in Toronto, Canada. As IASP prepares to release recordings of the World Congress' Plenary Lectures and select Topical Workshops, we are excited to bring you this accompanying webinar. Continuing Medical Education (CME) credits will be available to those who attend.
Attendance is free for IASP members, though registration is still required. A registration fee of $25 is required for non-IASP members. To become an IASP member, you can join here. Trainee memberships are $50 per year, while regular memberships are $180 or $230 per year, depending on income level.
Taking, Processing, Analyzing, Learning: Skin Punch Biopsies - More Than Just Fiber Count
One histological hallmark of neuropathies is the loss of intraepidermal nerve fibers, as shown by skin punch biopsy from the distal and proximal leg. While the procedure is fast and simple, even small but strategic inaccuracies may lead to clinically relevant misinterpretations. Following standardized guidelines for processing and analyzing skin samples is mandatory to ensure accurate data.
Advances in methodology have carried skin biopsies far beyond the mere determination of nerve fiber density. For example, it is now possible to distinguish between somatosensory and autonomic nerve fibers, to assess sweat gland innervation and quantify morphological abnormalities of the nerve fibers (such as axonal swellings), and to identify nerve fiber subtypes positive for calcitonin gene-related peptide (CGRP) or substance P.
This workshop will help teach the audience how to accurately obtain, process, and analyze skin punch biopsies for clinical and research purposes using short presentations and prerecorded videos.
Participants include:
-- Roy Freeman, M.B.Ch.B, Beth Israel Deaconess Medical Center and Harvard Medical School
Clinical and Research Aspects of Skin Biopsies
-- Pall R. Karlsson, PhD, Danish Pain Research Center, Aarhus University, Denmark
How to Take and Process a Skin Biopsy Following Published Guidelines
-- Eleonora Galosi, MD, PhD, Sapienza University of Rome, Italy
How to Stain and Analyze Skin Biopsies with Traditional and Unconventional Biomarkers
Learning Objectives:
1) Assess the theoretical and practical aspects on how to take, process, and analyze skin punch biopsies.
2) Assess structures and analyses that can be performed on the skin biopsies other than quantification of intraepidermal nerve fibers.
3) Compare the strengths and limitations of skin punch biopsies.