Over the past century and an half, human brain mapping consisted in pinning small functionally responsive areas within the brain. However the real extent of these areas and their eventual overlap remains unknown. The challenge now facing neuroscience is to define boundaries for functionally responsive areas at the group and the individual level. Many approaches parcellating the brain in areas with different features became recently available including post-mortem and in vivo architectonics, tractography-based connectivity, functional coactivation, and resting state functional connectivity. However, what these methods really measure and what conclusion can be drawn, are not yet fully clear to the scientific community. This course addresses this need and is intended for a large audience of research scientist (e.g. from beginner to advanced level).