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spheroONE - an automated high throughput spheroid isolation and manipulation technology by Cellenion Conventional (2D) monolayer culture fails to recapitulate the complexity of native tissue and organs. Recent advances in three-dimensional (3D) in vitro models, in particular spheroids and organoids, offer unprecedented means to replicate human tissues, organs and diseases in a petri dish. Indeed, tumor spheroids display hallmarks of solid tumor such as multicellular composition, spatial architecture, diffusion barrier, differential gene expression and drug resistance, rendering them the model of choice in anti-tumor drug screenings. To date, preparation of spheroids for high throughput screenings are poised to rely on the random aggregation of a cell suspension in overlay culture labware (ca. non-adherent multi-well plates) which raises concern about sample homogeneity and cellular debris scattering. Moreover, to yield reproducible and predictive assays, pre-selection of spheroids based on their size, morphology and intern organization is critical. Hitherto, there is no tractable automated and high throughput means to isolate and manipulate spheroids. By merging Scienion’s sciDROP NANO® low-volume dispensing in air technology and cellenONE® software, Cellenion has developed the spheroONE, an innovative platform to isolate, inspect and dispense large-particle such as 3D spheroids. Here, we highlight the capabilities of the platform by demonstrating our ability to isolate spheroids with high accuracy (up to 100% single spheroid per well) and sort them according to user-defined characteristics (size and morphology). The preparation of assay-ready plates by isolating and dispensing spheroids prepared in bulk has the potential to significantly accelerate drug screening processes and improve their predictability.
Standardizing organoids for high-throughput screenings by SUN bioscience Stem-cell-derived organoids are now routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell culture in solid extracellular matrices hampers the routine use of the organoids at the industrial scale. We present the fabrication of bioengineered cell culture devices and scalable and automated methods for suspension culture and real-time analysis of thousands of individual organoids of various origin. This talk will cover applications for anticancer drug tumoroid screenings, treatment response screenings for Cystic Fibrosis and large-scale imaged based analyses of the blood-brain barrier (BBB). We demonstrate integration by design for the full automation of organoid work-flows.
Thermo Fisher Scientific Amplitude Solution featuring Thermo Scientific’s Momentum Workflow Software The global scientific response to the COVID19 pandemic was tremendous.(e.g., diagnostics, screening of potential therapeutics and vaccine development research, viral biology, etc). However laboratories performing COVID-19 testing continue to face the combined challenges of rapidly scaling workflows to meet the demands of communities while ensuring the accuracy and reproducibility of results. This led to an increasing need for automated testing methodologies that not only provide sample to result tracking, but also increasing productivity and minimizing user intervention to meet industry needs. provided the opportunity to do more with robotic workflows. One such solution is the Thermo Fisher Scientific Amplitude Solution featuring Thermo Scientific’s Momentum workflow software, a highly automated molecular diagnostic testing solution that can analyze up to 8,000 COVID19 specimens in 24 hours. This is a standardized solution which provides a rapidly deployable and consistent solution for diagnostic labs worldwide In addition to the diagnostic systems which address active and ongoing COVID infection, automated serological assays lab automation systems are needed to detect the presence of antibodies in patent samples. This determines if a patient has been exposed to the virus and their level of immune response. Thermo Scientific Momentum automated ELISA systems have been deployed to provide vaccine researchers with critical information on antibody presence and titre to determine vaccine efficiency. This presentation will provide: •An overview of the Amplitude Solution, along with the critical data tracking and automated data interpretation that is required for diagnostic workflows. •Details about the simple to use dashboard style interface which enables novice-to automation lab technicians to operate the system with minimal hands-on time and direction. •Case systems which highlight complete data tracking against and end-to-end workflow for assessing response to a vaccine candidate.